ELN Stable Expression Cell Line (HFF-1)

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location: Home > Products > Stable Cell Lines > ELN Stable Expression Cell Line (HFF-1)
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  • 细胞图0 ELN Stable Expression Cell Line (HFF-1)-细胞图0
  • 质粒图0 ELN Stable Expression Cell Line (HFF-1)-质粒图0
  • ELN Stable Expression Cell Line (HFF-1) ELN Stable Expression Cell Line (HFF-1)-产品图
ELN Stable Expression Cell Line (HFF-1) Copy Product Info
Catalog#: YOE-L043
Size: 1*10^6
Culture method: 84%DMEM+15%FBS+1%Sodium Pyruvate
  • 细胞图0 ELN Stable Expression Cell Line (HFF-1)-细胞图0
  • 质粒图0 ELN Stable Expression Cell Line (HFF-1)-质粒图0
  • ELN Stable Expression Cell Line (HFF-1) ELN Stable Expression Cell Line (HFF-1)-产品图
ELN Stable Expression Cell Line (HFF-1) Copy Product Info
Catalog#: YOE-L043
Size: 1*10^6
Culture method: 84%DMEM+15%FBS+1%Sodium Pyruvate

Detailed Product Information

Cell Info

Catalog YOE-L043 Transcript NM_000501.4
Cell name ELN Stable Expression Cell Line (HFF-1) Species Human
Gene Name ELN Passage ratio 1:2
Cell Morphology Fibroblasts,adherent Antibiotic Concentration Puro=1.5μg/ml
Culture method 84%DMEM+15%FBS+1%Sodium Pyruvate Mycoplasma test negative
Catalog
YOE-L043
Cell name
ELN Stable Expression Cell Line (HFF-1)
Gene Name
ELN
Cell Morphology
Fibroblasts,adherent
Passage ratio
1:2
Transcript
NM_000501.4
Species
Human
Antibiotic Concentration
Puro=1.5μg/ml
Mycoplasma test
negative
Culture method
84%DMEM+15%FBS+1%Sodium Pyruvate

Product Description

This gene encodes a protein that is one of the two components of elastic fibers. Elastic fibers comprise part of the extracellular matrix and confer elasticity to organs and tissues including the heart, skin, lungs, ligaments, and blood vessels. The encoded protein is rich in hydrophobic amino acids such as glycine and proline, which form mobile hydrophobic regions bounded by crosslinks between lysine residues. Degradation products of the encoded protein, known as elastin-derived peptides or elastokines, bind the elastin receptor complex and other receptors and stimulate migration and proliferation of monocytes and skin fibroblasts. Elastokines can also contribute to cancer progression. Deletions and mutations in this gene are associated with supravalvular aortic stenosis (SVAS) and autosomal dominant cutis laxa.
Gene Expression Validation
No. Sample hELN-1CтMean β-h_actinCтMean ΔCтMean ΔΔCт Expression Rate
1 ELN Stable Expression Cell Line (HFF-1) 21.025 13.146 7.879 -13.381 10667.984
2 HFF-1_Ctrl(Puro) 35.084 13.824 21.260 0.000 1.000
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:HFF-1
STR Info (Cell bank)
Cell Line:HFF-1
Allele1Allele2Allele3Allele4Allele1Allele2Allele3
D5S818 11111111
D13S317 11131113
D7S820 91012.091012.0
D16S539 10121012
VWA 141517.019.0141517.0
TH01 799.3799.3
AMEL XYXY
TPOX 811811
CSF1PO 71112.013.071112.0
D12S391 171819.3
FGA 192122.025.0
D2S1338 171920.0
D21S11 282930.032.2
D18S51 101316.0
D8S1179 101415.0
D3S1358 151718.0
D6S1043 121317.0
PENTAE 71221.0
D19S433 131415.0
PENTAD 91213.016.0
D1S1656 121617.3
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

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    High expression
    Exclusive cell selection technique, ensuing high expression, RT-qPCR validation data provided.
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    Good stability
    Stable gene copy number, low passage, can be stably passaged for more than 20 passages.
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    Strict QC
    Strict quality control, ensuing no bacterial and mycoplasma contamination, STR authentication and cell post-thawing viability test passed; High standard delivery.

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